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| 名称： | anti- Tubulin-beta antibody |
|---|---|
| 货号： | FNab09105 |
| 适应种属 ： | 人源,大鼠,小鼠 |
| 规格： | 100ug(微克) |
| 状态 ： | 液体 |
| 纯化方法 ： | 蛋白A/G柱纯化 |
| 纯度： | 经SDS-PAGE分析,纯度大于95% |
| 宿主： | 小鼠 |
| 亚型： | IgG1 |
| 存储： | 用添加了0.02%叠氮化钠和50%甘油的PBS溶解,PH值7.3,可在-20℃下保存12个月（避免反复冻融） |
| 免疫原 ： | tubulin, beta 3 |
| 免疫原别称 ： | Beta tubulin antibody, MC1R antibody, TUBB antibody, Tubulin-beta antibody |
| 分子量： | 50 kDa |
| 验证应用： | ELISA, WB, IHC, IF, IP |
| 建议稀释比例： | WB: 1:10000-1:50000; IF: 1:20-1:200; IP: 1:500-1:2000; IHC: 1:50-1:500 |
| UniProt ID： | Q13509 |
| 克隆性： | 单克隆 |
| 背景介绍 ： | There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha and beta tubulins have been widely used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. |
| 图像 ： |  Immunofluorescent analysis of HepG2 cells using FNab09105 ( Tubulin-beta Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) 。  Immunohistochemistry of paraffin-embedded human brain tissue slide using FNab09105(Tubulin-beta Antibody) at dilution of 1:400。  IP Result of anti-Tubulin-beta (IP: FNab09105, 5ug; Detection: FNab09105 1:1000) with HeLa cells lysate 880ug。  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with FNab09105(Tubulin-beta Antibody) at dilution of 1:10000。 |
